HopsGuy Posted July 12, 2006 Posted July 12, 2006 They're remarkable little dynamos, and survive in some incredibally harsh environments. There's absolutely nothing they can't do, or can't be trained to do. 722074[/snapback] I can see it now. "Hi I'm Billy Mays for HaloPro Clean. Just sprinkle some of our patented Halobacterium Powder on your tub-tile-or-sink, turn on our LaserClean light bulbs, and let these guys go to work. Your place will be clean in no time.*" *HaloPro Clean is a wholly-owned subsidiary of ThisCrapDontWork Industries, LLC. No guarantee of success is implied. Offer not valid in the Western Hemisphere. Tax Title License Exra. Void where prohibited. Violates the 2nd Law of Thermodynamics. Perpetual Motion causes cancer in labratory animals.
Ramius Posted July 12, 2006 Posted July 12, 2006 Sorry man. I'm a microbiologist, and have a real soft spot for Arhcaea, which is really what Halobacterium are. Woese is me every time I hear them referred to as "bacteria." That being said, this a just a form of photosynthesis, like plants do, but without the chlorophyll. They're remarkable little dynamos, and survive in some incredibally harsh environments. There's absolutely nothing they can't do, or can't be trained to do. 722074[/snapback] Well, tell your !@#$ing bacteria to stop contaminating my stem cell cultures!
stuckincincy Posted July 12, 2006 Posted July 12, 2006 (edited) Actually, our original dispute is over the Second Law of Thermodynamics and your near-utter lack of reading comprehension. Which, frankly, makes you damned lucky I diminish myself by responding to your idiocy. 722070[/snapback] You are uniquely fit Next thing will be color happens because wavelengths are absorbed and one is reflected instead of promotion and release of a drop of that promoted election. Please keep responding - you are quite the amusement, and add to the forum. Whatever moniker you choose to use. If I'm comtemporary, it's CTM and the resurrected DC Tom...quite entertaining. Edited July 12, 2006 by stuckincincy
stuckincincy Posted July 12, 2006 Posted July 12, 2006 It's a membrane-bound protein, so you could conceivably pack them in pretty tight into some hydrophobic matrix. I would imagine they'd be pretty stable in a system like that, as proteins are quite happy under those conditions. The only problem I could see would be how much conformational change the protein needs to go through in order to get the desired effect. It is my understanding though, that the actual excitation/relaxation is done by the retinal molecule attached to the bacteriorhodopsin. It's the retinal that is light-sensitive, and it's the protein that facilitates pushing the protons across the cell membrane. If you're just looking for a color change, and not for proton translocation, you wouldn't need the protein to move that much, if at all. The retinal/chromophore color change is really all you'd be interested in. So you'd just need them packed in pretty tight in some crystalline/lattice monolayer. At least that was my understanding of the article. They've actually been working with these types of phototransducing molecules for a while in bioengineering and nanotech research. Bacteriorhodopsin was the first membrane protein they got a structure for, way back in the early 70's. They've done a fair amount of work using them in getting current through membranes, as they are proton pumps, after all. 722062[/snapback] You are going afield. Stick to your suppository studies..
Johnny Coli Posted July 12, 2006 Posted July 12, 2006 Well, tell your !@#$ing bacteria to stop contaminating my stem cell cultures! 722090[/snapback] They were here first!
Johnny Coli Posted July 12, 2006 Posted July 12, 2006 I can see it now. "Hi I'm Billy Mays for HaloPro Clean. Just sprinkle some of our patented Halobacterium Powder on your tub-tile-or-sink, turn on our LaserClean light bulbs, and let these guys go to work. Your place will be clean in no time.*" *HaloPro Clean is a wholly-owned subsidiary of ThisCrapDontWork Industries, LLC. No guarantee of success is implied. Offer not valid in the Western Hemisphere. Tax Title License Exra. Void where prohibited. Violates the 2nd Law of Thermodynamics. Perpetual Motion causes cancer in labratory animals. 722087[/snapback] You're not that far off, there, dude! Everyone is manufacturing "antibiotic" pillows and crap, when they ought to be seeding their environment with the right kinds of bugs. A lactobacilli a day, will keep the candida away.
stuckincincy Posted July 12, 2006 Posted July 12, 2006 (edited) Ahh... So long... Edited July 12, 2006 by stuckincincy
Fezmid Posted July 12, 2006 Author Posted July 12, 2006 Wow, who knew that a potential 50TB disk could cause such a thread... Some may be interested in the thread over at /. http://hardware.slashdot.org/article.pl?sid=06/07/11/209229
Beerball Posted July 12, 2006 Posted July 12, 2006 A lactobacilli a day, will keep the candida away. 722105[/snapback] Ahhhhhh, memories. My Grams used to tell me the same thing when she tucked me in at night.
Dan Gross Posted July 13, 2006 Posted July 13, 2006 Sorry man. I'm a microbiologist, and have a real soft spot for Arhcaea, which is really what Halobacterium are. Woese is me every time I hear them referred to as "bacteria." That being said, this a just a form of photosynthesis, like plants do, but without the chlorophyll. They're remarkable little dynamos, and survive in some incredibally harsh environments. There's absolutely nothing they can't do, or can't be trained to do. 722074[/snapback] Let me know when you've trained them to get me a beer from the fridge...
BillsFanNC Posted July 13, 2006 Posted July 13, 2006 Well, tell your !@#$ing bacteria to stop contaminating my stem cell cultures! 722090[/snapback] No pen/strep in your media?
Ramius Posted July 13, 2006 Posted July 13, 2006 No pen/strep in your media? 722263[/snapback] yah theres pen/strep in the media. it may be a fungus, but it sure as hell LOOKS like bacteria under the scope. The problem hasnt actually been with the culture, is been with the bioreactor system we have. The !@#$ers wont contaminate it right away either. The system is assembled and closed in the sterile hood, then transferred to the incubator. the part we cant figure out is that these bastards dont contaminate it until a week into the run.
kegtapr Posted July 13, 2006 Posted July 13, 2006 Wow, I'm out of my league here. So I'll just add, Erectile Dysfunction? Go Ingest Viagra Every Six Hours, Erection After Digestion.
Johnny Coli Posted July 13, 2006 Posted July 13, 2006 yah theres pen/strep in the media. it may be a fungus, but it sure as hell LOOKS like bacteria under the scope. 722504[/snapback] It's mycoplasma contamination.
Beerball Posted July 13, 2006 Posted July 13, 2006 It's mycoplasma contamination. 722557[/snapback] Bastard beat me to it! I knew it was my coplasma.
Ramius Posted July 13, 2006 Posted July 13, 2006 It's mycoplasma contamination. 722557[/snapback] i dont care what it is, i just want it to go away and stop interfering with my research!!!
Crap Throwing Monkey Posted July 13, 2006 Posted July 13, 2006 It's mycoplasma contamination. 722557[/snapback] I've had that before. It sucked.
Johnny Coli Posted July 13, 2006 Posted July 13, 2006 i dont care what it is, i just want it to go away and stop interfering with my research!!! 722559[/snapback] You're going to have to treat your cultures with cipro. Here's an article by Roche (Elimination of Mycoplasma Contamination in Cell Cultures(pdf)). Cipro is your best bet. They give your chances as being 75%, but I would think that's pretty optimistic. You'll have to play around with the cipro concentration because at high concentrations it's going to kill your cell line. It's also a pretty significant time investment, possibly weeks to make sure you don't just knock down their numbers to indetectable limits. They'll come back, and the ones that do will be resistant to cipro. Then there's no stopping them.
BillsFanNC Posted July 13, 2006 Posted July 13, 2006 You're going to have to treat your cultures with cipro. Here's an article by Roche (Elimination of Mycoplasma Contamination in Cell Cultures(pdf)). Cipro is your best bet. They give your chances as being 75%, but I would think that's pretty optimistic. You'll have to play around with the cipro concentration because at high concentrations it's going to kill your cell line. It's also a pretty significant time investment, possibly weeks to make sure you don't just knock down their numbers to indetectable limits. They'll come back, and the ones that do will be resistant to cipro. Then there's no stopping them. 722578[/snapback] JC is correct. I have had cultures get infected with mycoplasma before and it's a B word to get rid of. Thankfully I don't do much cell culture work anymore.
Crap Throwing Monkey Posted July 13, 2006 Posted July 13, 2006 You're going to have to treat your cultures with cipro. Here's an article by Roche (Elimination of Mycoplasma Contamination in Cell Cultures(pdf)). Cipro is your best bet. They give your chances as being 75%, but I would think that's pretty optimistic. You'll have to play around with the cipro concentration because at high concentrations it's going to kill your cell line. It's also a pretty significant time investment, possibly weeks to make sure you don't just knock down their numbers to indetectable limits. They'll come back, and the ones that do will be resistant to cipro. Then there's no stopping them. 722578[/snapback] I just want to say that you're wrong. Just because, it wouldn't be a thread on TSW if some !@#$ing know-nothing dumbass didn't tell the professionals they were wrong...
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