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Wacka

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Everything posted by Wacka

  1. You libs laugh at that like it was a joke. If he would have choked to death, Cheney would have been president, which I gather from your vitriol against him would have been your worst nightmare.
  2. I was in molecular biology, but now I do environmental due-diligence. Cloning a gene is actually inserting it in a circle of DNA (the circles are called plasmids), then getting it into a bacteria so the gene can be expressed, or get more copies (by growing the bacteria). Originally, you took cells, treated them with certain solutions and the plasmids, heat shocked them for a few minutes , let them grow for an hour and then plate them. With electroporation (started being done about 10 years ago or so if I remember right), you treat the cells with a solution, put them in a little disposable chamber with the DNA. Push a button (zap the cells), and plate the cells. There are controllers that can vary the length of charge, decay rate and other parameters. If there is too much salt in the solutions, it arcs and makes a little pop and doesn't work. Apoptosis is what gets rid of the webbing between your toes and fingers before you are born. When it doesn't work, you are born with webbed feet (supposedly Dan Akroyd was). Amplifying DNA (using PCR) is pretty easy too.
  3. Wishful thinking. The rats are already jumping ship.
  4. This is known as electroporation. I have used it with bacteria. Nice pop and flash if it arcs when you do it. Apoptosis is the process that an organism uses to get rid of damaged cells.
  5. The Messiah can't even put two sentences together without inserting an "uh". He had to use a teleprompter to introduce a high level nominee the other day. God, this guy can't say anything that wasn't written for him. Why do you think he turned down the town hall style of debates that McCain offered him.
  6. These idiots cant get out of their own way. Hillary meets with the Russian Foreign Minister today in Geneva. She gave him a "push button" (I guess like the button for office supplies). This was to symbolize the resetting of relations between the US and them. She said the button said reset in Russian. The Foreign Minister said "No. It actually says overpay." :lol: Couldn't they ask someone in the State Department that knows Russian, to get the correct word?
  7. Yes. I read he was diving in a swimming pool in college and hit the bottom. Became a parapelegic.
  8. Gupta withdraws from consideration Is his next choice Dr. Dean Edell? Dr. Phil? Dr. Laura?
  9. I've driven by there once. I've heard that if you eat at the restaurant there, it's some of the best beef in the state. probably because it's so fresh. It's almost as if you could say " I want a T-bone from THAT one.
  10. My grandfather wasn't an alcoholic, but almost every day, he'd walk around the corner in Kaisertown after lunch and dinner for a shot and a beer. It got him out of the house. He did it until a month or two before he died at the age of 94. If my mother went to visit and didn't get an answer at the door, she'd check one of the bars.
  11. Like the Nuge says: Kill it and Grill it!!!
  12. Why is it stupid when the President is snubbing one of our biggest friends?
  13. First, the Messiah returns a bust of one of the great statesmen of the 20th century, Winston Churchill to Great Britain, but then when given a pen set from the HMS Gannet and a first edition biography of Churchill, he gives PM Brown a DVD set, that's right, a DVD set of 25 great American movies. He also had the British press stand out in the cold for close to an hour and cancels a press conference with Brown. Way to treat on of our best friend in the world.
  14. Only took 1 post for someone to say "Sign him!"
  15. Coke can hurt you years later.
  16. He's already got that Mussolini look down pat.
  17. 800 /1000 on this and the Hot or Heavy quiz
  18. Can't blame me for back then, I voted for Ford.
  19. Retreats even work on a small level. When I was working at Stanford, our department went on one to in Monterey, CA. We heard what all the other labs were doing and it produced some productive collaborations.
  20. What are you on?
  21. Breaking Bad starts again next Sunday, I think.
  22. He also told people to "Obama Proof" their investments.
  23. Israel went in there to knock out the sites that have been lobbing missles at them DAILY. Every day this administration tops itself in idiocy.
  24. That is until the people getting their taxes raised don't have the money to invest in the company you work for and you get laid off.
  25. It's an Oliver Stone movie. None of it is accurate.
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